TABLE 1.

PCR primers and conditions used in this study

Primer designation	Primer sequence (5′—3′)	Target	PCR condition						Length of PCR product (bp)	Reference
			Denaturing		Annealing		Extension
			Temp (°C)	Time (s)	Temp (°C)	Time (s)	Temp (°C)	Time (s)
K260	GAG CGA ATA TTC CGA TAT CTG GTT	selC	94	90	60	90	72	120	2,173	65
K295	CGC CGA TTT TTC TTA GCC CA
K255	GGT TGA GTC GAT TGA TCT CTG G	selC-LEE left junction	94	90	60	90	72	120	405	65
K260	GAG CGA ATA TTC CGA TAT CTG GTT
K295	CGC CGA TTT TTC TTA GCC CA	selC-LEE right junction	94	90	60	90	72	120	418	65
K296	CAT TCT GAA ACA AAC TGC TC
SK1	CCC GAA TTC GGC ACA AGC ATA AGC	eae	94	30	52	60	72	60	863	49
SK2	CCC GGA TCC GTC TCG CCA GTA TTC G
ANK-49	ATG TTA TCC TCA TAT AAA ATA AAC	pas	94	20	50	60	72	120	1,221	21
ANK-50	TTA ATA CGA CAG TGG AAT ATG
escC-U	AGA CAA CAC CAG GGA CGA C	escC	94	60	56	60	72	90	575	This study
escC-L	ACA AGC TGC CCC GTC CTC T
escF-U	ATG GCG GAT TGA GAC ACC T	escF	94	60	52	60	72	90	518	This study
escF-L	TCA ACA TTC CAC TTT CTA CG
espI-I	ATG GAC AGA GTG GAG ACA G	espI	94	30	52	60	72	60	560	This study
espI-II	GCC ACC TTT ATT CTC ACC A
iha-I	CAG TTC AGT TTC GCA TTC ACC	iha	94	30	56	60	72	90	1,305	This study
iha-II	GTA TGG CTC TGA TGC GAT G
btuB-I	GCC CCT TCC CAC TGT TTA CT	btuB	94	30	55	60	72	90	1,032	This study
btuB-II	GGT ATT GAT TGA TGG AGT GCG
espI-B	GAG AAA GAC TGG AAA AAT CAA G	espI-iha	94	30	54	60	72	240	3,916	This study
iha-A	GGC AAG GAC AAC CCC ATC T
iha-B	CGT GAT GGT GAT AAC AAA GG	iha-btuB	94	30	54	60	72	90	1,325	This study
btuB-A	CGG AAA AGA GTA AAC AGT GG