Figure 3.

EGFRvIII-DBTE induces T cell activation

(A) Fluorescent images of U87vIII cells in a tumor-killing assay upon addition of mouse sera treated with EGFRvIII-DBTE or pVAX1. Day 14 sera were used. Target cells are shown in green. CD69 activation is shown in red. Caspase-3 induction is shown in blue. (B–D) Quantified GFP⁺ cell counts, CD69 activation, and caspase-3 induction in the tumor-killing assay. Flow cytometry data showing (E) IFN-γ, TNF-α, IL-2, and (F) CD107a responses in CD4⁺ T cells and CD8⁺ T cells in a 24-h tumor-killing assay. (G) Tumor-killing assay with CD4⁺ T cells and/or CD8⁺ T cells in the presence of EGFRvIII-DBTE. (H) Fluorescent images of the target cells at 0-, 6-, and 24-h time points.