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In vivo expression of EGFRvIII-DBTE
(A) T cell-mediated cytotoxicity assay against U87vIII cells using NSG mouse sera treated with a single injection (100 μg) of pVAX1, EGFRvIII-DBTE, or recombinant EGFRvIIIxCD3 antibody. (B) T cell-mediated cytotoxicity assay against U87vIII cells using NSG mouse sera treated with various doses of EGFRvIII-DBTE. % Cytolysis data after 48-h incubation were plotted for both (A) and (B).
