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. 2022 Jun 9;45:101–115. doi: 10.1016/j.jare.2022.05.011

Fig. 3.

Fig. 3

Influence of deep-sea bacteria on the health of mice. (A) Scatter plots of the value of 16 physiological parameters of control mice and experimental mice. Both control mice and experimental mice consisted of 3 female and 3 male ICR mice. Each of the experimental mice was fed with the bacteria isolated from a deep-sea sediment, while the control mice were fed with sterilized water. In total, 106 deep-sea sediments were used. Subsequently the physiological parameters of the experimental and control mice were examined. Each dot represented the mean of a physiological parameter of 6 mice (3 female and 3 male mice). As an example, ALT of experimental mice was enlarged. In the enlarged image, each line indicated the mean (on the right) of 6 ALT values of the mice treated with the bacteria from a deep-sea sediment (on the left). As shown in the enlarged image, the ALT values of 6 mice were close to each other, showing that there was no difference of physiological parameters between 6 mouse individuals. BW, body weight; BASO, basophilic granulocyte; RBC, red blood cell; PLT, platelet; NEUT, neutrophil; MONO, monocyte; MCV, mean corpuscular volume; MCHC, mean corpuscular hemoglobin concentration; MCH, mean corpuscular hemoglobin; LYMPH, lymphocyte; Hct, hematocrit; Hb, hemoglobin; EO, eosinophil; WBC, white blood cell, ALT, alanine aminotransferase; GLU, glucose. (B) Volcano plots showing statistical significance (y axis) versus fold change (x axis) of each physiological parameter of mice after administration of the bacteria from each deep-sea sediment sample. The colored points above the horizontal dashed line indicated the significant changes with p < 0.05 (lower line) and p < 0.01 (upper line). The percentage of BASO was 0% in most of the samples. Therefore it was not shown. (C) Influence of deep-sea bacteria on the number of tuft cells in the small intestine of mice. The small intestines of control and experimental mice were labeled with the antibody against DCLK1 (green), the marker protein of tuft cells. The nuclei were stained with DAPI (blue). The tuft cells were indicated with arrows. Scale bar, 100 μm. (D) The expression level of IL-25 in the small intestine tissues of control and experimental mice. Quantitative real-time PCR was used to examine the expression profiles of IL-25 (**, p < 0.01). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)