Table 5.

Intra-plate and inter-plates precision calculation.

Intra-plate precision
Lot	Sample	Screening assay (SNR values)			Confirmatory assay (%Inhibition)
		HPC	LPC	NC	HPC	LPC	NC
38	1	12.91	1.74	1.02	87.91	41.36	6.47
	2	12.15	1.54	0.97	87.20	36.93	2.76
	3	11.14	1.35	0.99	86.64	27.82	−3.20
	4	*	1.42	1.00	81.98	31.53	2.69
	5	9.95	1.59	1.01	84.19	37.09	8.70
	Mean	11.54	1.53	1.00	85.58	34.95	3.48
	STD	1.28	0.15	0.02	2.45	5.29	4.53
	%CV	11.09	9.80	2.00	2.86	15.14	/
Robustness
39	1	11.02	1.65	1.01	87.76	40.56	6.39
	2	10.41	1.58	1.00	86.32	37.66	0.99
	3	9.44	1.59	1.01	83.85	39.56	6.78
	4	9.74	1.56	1.03	84.75	36.79	−0.48
	5	10.20	1.61	0.99	85.58	38.26	2.91
	Mean	10.09	1.60	1.01	85.65	38.57	3.32
	STD	0.65	0.03	0.01	1.50	1.50	3.22
	%CV	6.44	1.88	0.99	1.75	3.89	/
40	1	9.09	1.58	1.00	84.98	37.81	−6.97
	2	9.21	1.56	1.03	83.78	37.14	6.14
	3	7.62	1.36	0.95	81.25	29.94	4.72
	4	7.65	1.48	1.01	82.54	32.07	4.44
	5	7.76	1.40	1.01	82.10	30.93	8.97
	Mean	8.16	1.48	1.00	82.93	33.58	3.46
	STD	0.85	0.10	0.03	1.47	3.64	6.10
	%CV	10.42	6.76	3.00	1.77	10.84	/
38–40	Mean	9.88	1.53	1.00	84.72	35.70	3.42
	STD	1.60	0.11	0.02	2.17	4.15	4.41
	%CV	16.19	7.19	2.00	2.56	11.62	/
Inter-plates precision
41	1	9.44	1.48	1.00	85.90	29.21	−1.29
	2	8.64	1.55	1.04	85.96	33.97	0.62
	3	8.18	1.51	1.01	84.47	34.44	4.96
42	1	11.48	1.60	0.97	89.15	40.92	3.82
	2	10.77	1.57	0.97	88.18	34.92	2.85
	3	9.89	1.53	1.01	87.37	36.44	6.26
43	1	7.47	1.51	1.01	82.25	33.59	6.73
	2	7.83	1.39	1.00	84.45	29.44	2.97
	3	7.04	1.35	1.04	82.56	27.19	3.59
47	1	9.05	1.60	1.04	83.95	34.08	6.40
	2	9.42	1.60	1.01	84.38	35.80	2.65
	3	8.66	1.46	0.98	84.27	33.79	0.68
49	1	10.09	1.73	0.97	82.31	44.95	2.70
	2	10.55	1.68	0.96	83.37	42.19	0.68
	3	9.39	1.87	0.97	77.92	49.55	−0.13
38–43, 47 & 49	Mean	9.52	1.55	1.00	84.58	35.86	3.16
	STD	1.45	0.12	0.02	2.44	5.11	3.52
	%CV	15.23	7.74	2.00	2.88	14.25	/

Note: *As the CV value of duplicate wells exceeded 20%, the assay data were removed from the precision calculation.