Fig. 8.

Anti-scarring phenotypes of mouse corneas treated with human CSSCs after quality test by miR-29a expression in EVs. (A) By qPCR, injured corneas treated with HC540 and 641 (high miR-29a expression) had significantly downregulated expression of fibrosis genes (αSMA, Col3A1 and FN), when compared to corneas after HC618 and 572 cell treatment (low miR-29a expression) and wound controls (triplicate run with single cornea each sample; *P < 0.05; Mann-Whitney U test). (B) Immunofluorescence showed suppressed expression of fibrosis markers (FN, αSMA and CD90/Thy1) in mouse corneas treated by HC540 and 641 cells (n = 3 each). In contrast, wounded corneas without treatment or treated with HC618 and 572 showed stronger immunoreactive signals. Scale bars: 20 μm.