. Author manuscript; available in PMC: 2023 Mar 11.

Published in final edited form as: J Vis Exp. 2022 Jul 12;(185):10.3791/63923. doi: 10.3791/63923

Table 1: Collection parameters for several cryo-ET studies.

Studies targeting the reconstruction of molecular detail from purified or in situ proteins compared to a study aiming to resolve and segment ultrastructural cellular features ^17,42,45,47.

Sample	Cryo-ET type	Å/px	Tilt range (+/−)	Tilt step (°)	Defocus range (μm)	Total dose (e⁻/Å²)	Resolution	Raw data	Reference
Apoferritin	Purified/Molecular (STA)	1.34	60	3	1.5 – 3.5	102	2.86	EMPIAR-10787	17 & this paper
HIV-1 Gag	Purified/Molecular (STA)	1.35	60	3	1.5 – 3.96	120	3.1	EMPIAR-10164	17
Ribosome	Purified/Molecular (STA)	2.1	60	3	2.2 – 4.3	120	7	EMPIAR-10304	42
SARS-CoV-2 spike	Lamella/Molecular (STA)	2.13	54	3	2 – 7	120	16	EMPIAR-10753	45
Neuron axon structure	Cellular (ultrastructural)	5.46	60	2	3.5 – 5	90	N.D.	EMPIAR-10922	47