TABLE 1.
Strains and plasmids
| Strain or plasmid | Description |
|---|---|
| V. vulnificus strains | |
| M06-24/O,a M06-24/T, LC4/O, LC4/T, E4125/O, and E4125/T | Opaque- and translucent-phase variants of clinical isolates |
| 345/O and 345/T | Phase variants of environmental isolate |
| CVD752 | Acapsular TnphoA mutant of M06-24/O (insertion in wzaVv) |
| CVD737 | Acapsular TnphoA mutant of M06-24/O (insertion in ORF4) |
| M06-24/31T and M06-24/32T | Nonpolar mutant of M06-24/O (insertion in wzaVv) |
| Plasmids | |
| pACW15 | Partial wzaVv gene cloned into pBluescript II |
| pACW29 and pACW30 | pACW15 with in-frame or +1 nonpolar insertion in wzaVv |
| pACW31 and pACW32 | wzaVv gene from pACW29 and/or pACW30 cloned into pRK404 |
| pACW36 | Intact wzaVv gene cloned into pGEM-TEasy |
| pWZA1 | Intact wzaVv gene cloned into pRK404 |
| pWZA2 | Intact wzaVv gene with upstream sequences cloned into pRK404 |
| pR751 | IncP plasmid |
a
O, opaque; T, translucent.