Table 2.

Immersion solution preparation and immersion protocol.

Immersion Solution	Brand Name or Manufacturer	Composition	Preparation and Immersion Procedure
Distilled water	Distilled water	------	Immersion throughout the experiment at room temperature
Effervescent tablet (Fittydent alkaline peroxides)	Fittydent super (Fittydent International GmbH, Vienna, Austria)	Polyvinylpyrrolidone, sodium bicarbonate, sodium carbonate peroxyhydrate, potassium monopersulfate, sulfamic acid, sodium perborate monohydrate, sodium lauryl sulfate, tetraacetylethylenediamine, aroma, color C.I. 42090	One tablet dissolved in 200 mL warm tap water (40 °C) for 5 min Repeated 180 times
Sodium hypochlorite	Sodium hypochlorite solution	Sodium hypochlorite solution, 1% active chlorine	(a) Solution of 5.25% sodium hypochlorite (1:5 dilution) diluted to obtain 1% sodium hypochlorite by adding 50 mL sodium hypochlorite to 200 mL water (b) Immersion for 10 min at room temperature Repeated 180 times