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Changes in the donor emission intensity in FRET experiments upon addition of 10 μg of LBP (180 nM) (A) or additional F515 LPS (10 μM) (B) to double-labeled liposomes composed of different phospholipids (10 μM). PS-PC and PS-PE are equimolar mixtures, and PL is composed of PC, PE, SM, and PS in molar ratios of 38.1:27.3:19.4:15.2. Changes in donor intensity are plotted against the average number of net negative charges per lipid molecule.
