B. avium strains |
|
|
4169 |
Wild type |
34 |
4169rif |
Spontaneous Rifr mutant of 4169 |
This study |
Pho20 |
4169rif bhuR::TnphoA
|
5 |
Pho20(fur) |
Pho20, fur gene interrupted by insertional integration of ptr5-1 |
This study |
E. coli strains |
|
|
H1717 |
Chromosomal lacZ under the control of a strong Fur-regulated promoter |
40 |
MGIQ1 |
K-12 derivative; lacIq1 Kanr
|
V. J. Hernandez |
AF633 |
MC4100 λφ(uspB-lacZ) |
11 |
Plasmids |
|
|
pCOS5 |
Cosmid vector; Ampr Tetr
|
4 |
p1016 |
45-kbp fragment of 4169 chromosome encoding the rhuI-rhuR-bhuR locus ligated into pCOS5 |
Murphy and Connell, unpublished |
pMECA |
Cloning vector, Ampr
|
W. A. Parrott |
pERM1 |
3-kbp EcoRI fragment of p1016 ligated into pMECA |
Murphy and Connell, unpublished |
pBluescript KS |
High-copy-number expression vector; Ampr
|
Stratagene |
pAEK10 |
199-bp rhuR-bhuR intergenic region ligated into pBluescript KS |
This study |
pAEK13 |
142-bp rhuI promoter region ligated into pBluescript KS |
This study |
p1910 |
Mobilizable ColE1 replicon; Ampr
|
S. Stibitz |
ptr5-1 |
Truncated fur with the ATG start codon mutated to TGA; mutant gene ligated into p1910 |
29 |
pMGJ66 |
lacIq1-containing plasmid; Tetr
|
M. G. Jobling |
placI
|
lacIq1 allelle from pMGJ66 ligated into pUFR047 |
This study |
pET21a |
Expression vector; Ampr
|
Novagen |
pFI-7 |
rhuI ORF ligated into pET21a |
This study |
pRK415 |
Bordetella spp. expression vector, Tetr
|
19 |
pERM26 |
rhuI ORF from pFI-7 ligated into pRK415 |
This study |
pERM26.4 |
rhuI frameshifted at the XhoI site ligated into pRK415 |
This study |
pRK415Δ |
Alpha peptide gene in pRK415 frameshifted at the XhoI site |
This study |
pRS415 |
Promoter reporter vector; Ampr
|
38 |
pDJM31 |
rhuR-bhuR intergenic region ligated into pRS415 |
This study |
pUFR047 |
E. coli/Bordetella spp. shuttle vector; Ampr Gmr
|
6 |
pDJM41 |
Fragment of pDJM31 containing the tandem transcription terminators, the rhuR-bhuR intergenic region, and the lacZYA locus ligated into pUFR047 |
This study |