Figure 2. Expression levels of genes and proteins of Bax and Bcl-2 in the experimental groups. Findings of reverse transcriptase real-time polymerase chain reaction (PCR) for mRNAs of Bax (A); Bcl-2 (B); and Bax/Bcl-2 ratio (C) (** P < 0.01, *** P < 0.001). Immunoblots of Bax and Bcl-2 (D); from hippocampal cell lysates. Densities of Bax and Bcl-2 protein bands in the experimental groups are presented (E). The bar graph shows the mean ± SD (one-way ANOVA and Tukey's test, P < 0.05) (* P < 0.01, # P < 0.01). Abbreviations: C, control rats; D, diabetes rats; S, seed extract-treated rats; DH, harmine-treated diabetic rats; H, harmine-treated rats; DS, seed extract-treated diabetic rats; NS, non-significant.
