Skip to main content
. 2023 Mar 11;363:114379. doi: 10.1016/j.expneurol.2023.114379

Fig. 1.

Fig. 1

SARS-CoV-2 pseudotyped lentivirus did not infect β-III tubulin-positive neurons.

(A) Pseudotyped lentivirus of the original SARS-CoV-2 strain was added to hiPSC-derived neurons and stained with β-III tubulin (red), a neuronal marker. No expression of EGFP, a reporter protein that indicates the establishment of infection, was observed. Nuclei were counterstained with Hoechst (blue). Scale bar: 50 μm.

(B) Pseudotyped lentivirus of the Delta variant was added to hiPSC-derived neurons and stained with β-III tubulin (red), a neuronal marker. No expression of EGFP, a reporter protein that indicates the establishment of infection, was observed. Nuclei were counterstained with Hoechst (blue). Scale bar: 50 μm.

(C) Pseudotyped lentivirus of the Omicron variant was added to hiPSC-derived neurons and stained with β-III tubulin (red), a neuronal marker. No expression of EGFP, a reporter protein that indicates the establishment of infection, was observed. Nuclei were counterstained with Hoechst (blue). Scale bar: 50 μm.