Fig. 3.

Pseudotyped lentivirus of SARS-CoV-2 did not infect NS/PCs in cerebral organoids.

(A) Cerebral organoids were prepared to examine the infectivity of pseudotyped lentivirus of SARS-CoV-2 to NS/PCs. A representative image of paraffin sections stained with hematoxylin-eosin. Scale bar: 100 μm.

(B) EGFP, a reporter of the pseudotyped lentivirus of the original strain of SARS-CoV-2, was not detected in nestin-positive cells (red), a marker for NS/PCs. Nuclei were counterstained with Hoechst (blue). Scale bar: 20 μm.

(C) EGFP, a reporter of the pseudotyped lentivirus of the Delta variant, was not detected in nestin-positive cells (red), a marker for NS/PCs. Nuclei were counterstained with Hoechst (blue). Scale bar: 20 μm.

(D) EGFP, a reporter of the pseudotyped lentivirus of the Omicron variant, was not detected in nestin-positive cells (red), a marker for NS/PCs. Nuclei were counterstained with Hoechst (blue). Scale bar: 20 μm.