Figure 3.

Multiple dosing with a PNMC attenuates stress-induced hypersensitivity and priming to a NO donor. A, Stress paradigm and dosing regimen are shown. B, C, Following 3 d of repeated stress, female ICR mice were administered FeTMPyP (30 mg/kg, i.p.) or vehicle at 1, 24, 48, and 72 h poststress and tested for acute facial hypersensitivity (B) and grimacing (C). Upon returning to baseline thresholds, mice were checked for priming to low-dose SNP (0.1 mg/kg, i.p.). Stress-induced acute mechanical hypersensitivity and grimace responses in mice that received multiple injections of vehicle; however, these effects were attenuated by multiple injections of FeTMPyP, determined by a two-way ANOVA with Bonferroni’s post hoc analysis. *Significance between stressed mice that received FeTMPyP and those that received vehicle. All control groups received vehicle and were administered SNP before the priming phase (n = 6 for all groups). Data are represented as the mean ± SEM. Table 1, see for F-values. *p < 0.05, **p < 0.01, ****p < 0.0001.