Table 3.

Comparison of stress-induced TG mitochondrial activity within sex

	Metabolic parameters (pmol/min/100,000 cells)
	24 h		14 d
	Control	Stress	Control	Stress
Female
Basal respiration	7.21 ± 1.50	12.56 ± 1.41	12.68 ± 0.79	15.15 ± 2.06
Maximal respiration	16.14 ± 2.99	34.38 ± 5.91	27.59 ± 5.59	52.57 ± 6.50*
Spare reserve capacity	8.93 ± 2.89	21.82 ± 6.27	11.58 ± 2.25	34.08 ± 1.64**
Non-mitochondrial  respiration	8.14 ± 0.88	9.77 ± 2.52	3.03 ± 0.90	5.74 ± 1.20
Proton leak	3.22 ± 0.84	3.82 ± 0.42	4.63 ± 0.82	4.98 ± 0.49
ATP production	3.99 ± 0.66	8.74 ± 1.64	8.05 ± 1.07	23.50 ± 1.24***
Coupling efficiency	57.67 ± 1.25	68.75 ± 5.86	64.03 ± 4.76	69.70 ± 8.34
Male
Basal respiration	6.22 ± 0.19	13.78 ± 2.25	11.43 ± 0.27	11.25 ± 1.45
Maximal respiration	21.69 ± 3.05	45.29 ± 3.05**	23.59 ± 0.93	27.14 ± 0.39*
Spare reserve capacity	15.47 ± 3.06	31.51 ± 1.24*	12.36 ± 0.84	15.62 ± 1.78
Non-mitochondrial  respiration	4.26 ± 0.46	4.96 ± 2.09	4.08 ± 0.46	6.42 ± 1.50
Proton leak	2.85 ± 0.17	6.63 ± 2.13	0.73 ± 0.18	1.79 ± 0.36
ATP production	3.37 ± 0.31	7.15 ± 2.46	10.84 ± 0.05	12.27 ± 0.42
Coupling efficiency	54.65 ± 4.11	48.69 ± 14.91	93.75 ± 1.55	91.81 ± 0.40

Seahorse metabolic data are presented for female and male mice at 24 h and 14 d poststress. A two-tailed Welch’s t test found a significant increase in maximal respiration, spare respiratory capacity, and ATP production in stressed female mice compared with controls at 14 d poststress. Similarly, an increase in maximal respiration and spare respiratory capacity was identified in stressed male mice at 24 h poststress followed by an increase in maximal respiration between conditions at 14 d poststress. Data are represented as the mean ± SEM.

*p < 0.05, **p < 0.01, ***p < 0.001.