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. 2023 Mar 1;43(9):1614–1626. doi: 10.1523/JNEUROSCI.1922-22.2022

Figure 2.

Figure 2.

Reduced expression of β-spectrin enhances α-synuclein neurotoxicity. A, Climbing activity in control and α-synuclein transgenic Drosophila with and without reduced expression of β-spectrin. n = minimum of 60 flies per genotype (six biological replicates of 10 flies each). B, C, Brain degeneration assayed by hematoxylin staining in the anterior medulla of control and α-synuclein transgenic Drosophila with and without reduced expression of β-spectrin; n = 6. D, E, Immunostaining for TH in the anterior medulla of control and α-synuclein transgenic Drosophila with and without expression of β-spectrin. n = 6. All flies are 10 d old. β-spectrinKD flies are hemizygous for the complete loss-of-function mutation β-specem21, rescued to viability with transgenic expression of the β-specKpn+3 variant, which accumulates at significantly reduced levels. Controls are nSyb-QF2, nSyb-GAL4/+. Scale bars: B, 10 µm; D, 5 µm. Data are mean ± SEM. p values determined with one-way ANOVA with Bonferroni post hoc test (see Extended Data Fig. 1-1).