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. 2023 Mar 1;43(9):1614–1626. doi: 10.1523/JNEUROSCI.1922-22.2022

Figure 3.

Figure 3.

α-Synuclein interacts directly with β-spectrin, but not α-spectrin. A, Human α-synuclein and Drosophila β-spectrin interact in Ni-NTA and GST pull-down assays as monitored by immunoblotting for β-spectrin (His) or α-synuclein. B, No interaction of human α-synuclein and Drosophila α-spectrin in Ni-NTA or GST pull-down assays as monitored by immunoblotting for β-spectrin (His) or α-synuclein. C, Human α-synuclein and human βII-spectrin interact in Ni-NTA and GST pull-down assays as monitored by immunoblotting for βII-spectrin (His) or α-synuclein. D, No interaction of human α-synuclein and human αII-spectrin in Ni-NTA or GST pull-down assays as monitored by immunoblotting for αII-spectrin (His) or α-synuclein. E, Immunoprecipitation of α-synuclein in control and α-synuclein transgenic flies shows an association between α-synuclein and β-spectrin. Flies are 10 d old.