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. 2023 Mar 10;11(3):e005583. doi: 10.1136/jitc-2022-005583

Figure 2.

Figure 2

Anti-VEGF treatment improves the infiltration of EGFRvIII-CAR-T cells. (A) Intratumoral distribution of EGFRvIII-CAR-T cells inside of GFP-GSC005 GBM tumors imaged by multiphoton microscopy. Images were taken 24 hours after the injection of CAR-T cells (fluorescently labeled as red). Scale bars=50 µm. (B) Measurement of CAR-T cell (pink) number in GFP-GSC005 tumors (green) treated with PBS or B20 (2.5 mg/kg). scale bar=50 µm. (C) Percentage of EGFRvIII-CAR-T cells inside of GSC005 GBM tumors measuring by flow cytometry. Error bars show ±SEM. Statistical analysis was performed using Student’s t-test. *p<0.05, **p<0.01. CAR, chimeric antigen receptor; GBM, glioblastoma. PBS, phosphate buffered saline.