FIG. 3.

Effect of PDTC on Mono Mac 6 cells. Mono Mac 6 cells were treated with PDTC for 10 h or were left untreated. (A) Western blot analysis using antibodies against caspase-3 (top) or PARP (bottom). The caspase-3 and PARP antibody stainings were developed with a secondary antibody conjugated to horseradish peroxidase followed by visualization using an enhanced chemiluminescence kit as described in Materials and Methods. Data are representative examples of three similar experiments. (B) Flow cytometric analysis of apoptotic cells using annexin V-FITC. Cells were incubated with annexin V-FITC in a buffer containing propidium iodide (PI) and were analyzed by flow cytometry. PI-negative cells were gated and shown as histograms. (C) Detection of apoptosis in Mono Mac 6 cells by TUNEL reaction. The TUNEL reaction was performed as described in Materials and Methods. Fluorescence pictures were taken on a Zeiss axiophot 2 microscope. Data are representative examples of three similar experiments.