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. 2023 Mar 11;12(1):13. doi: 10.1038/s41389-023-00453-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A WB analysis of the half-life of BRCA1 protein in the indicated cells. GAPDH served as a loading control. B WB analysis of the polyubiquitin levels of BRCA1 in the indicated cells. C WB analysis of the polyubiquitin levels of BRCA1 in the indicated cells. D Co-IP assay revealing that endogenous TRIM47 interacted with endogenous BRCA1 in the indicated cells. E Immunoprecipitated Flag-tagged BRCA1 was gel-purified, transferred to a membrane, and incubated with His-tagged recombinant TRIM47, then detected using an antibody specific for His or Flag.