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. 2023 Mar 11;6:261. doi: 10.1038/s42003-023-04619-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Diagram showing the schematic used to obtain conditional deletion of SMAD2 and SMAD3 in the uterine epithelium using Ltf-cre. b–h Confirmation that effective deletion of the Smad2 and Smad3 floxed exons and protein levels were decreased in the uterine epithelium of Smad2/3 cKO mice at the mRNA level (b, c, n = 4 per genotype) and in protein lysates from purified epithelium (d, n = 3 per genotype). Lanes in (d) were generated from the same blot, which was sequentially probed and stripped with each of the indicated antibodies. e–h Immunohistochemical analysis of phosphorylated SMAD2 and SMAD3 (pSMAD2/3) in uterine cross-sections of control (n = 3) and Smad2/3 cKO mice (n = 3). Red arrows (f) show positively stained cells in uterine epithelial cells of controls, while yellow arrows (h) highlight the unstained epithelial cells in Smad2/3 cKO uterus. Histograms represent mean ± SEM analyzed by an unpaired two-tailed t-test.