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. 2023 Mar 11;6:261. doi: 10.1038/s42003-023-04619-2

Fig. 5. Gene expression profiling of endometrial organoids reveals that inhibition of SMAD2/3 signaling increases retinoid- and BMP-signaling pathways.

Fig. 5

RNA-sequencing of the endometrial epithelial organoids was performed to identify the gene expression differences between control and Smad2/3 cKO organoids. a Differentially expressed genes identified by RNAseq between the various organoid groups (Control + A83-01 vs. Control and Smad2/3 cKO vs. Control). b, c Volcano plots highlighting gene-level differences identified by RNAseq between Control + A83-01 vs. Control (b) and Smad2/3 cKO vs. Control (c) organoids. RNAseq data represent differentially expressed genes from four different mice per group, >1.4 fold or <0.714 fold change, FDR < 0.01. d, e Gene ontology analyses of overexpressed genes in Smad2/3 cKO organoids indicates that “Retinol Metabolism” pathway genes are overrepresented in Smad2/3 cKO organoids (d), while pathways related to “WNT/β-catenin” and are downregulated (e). f qPCR validation of differentially expressed genes identified by RNAseq in an independent set of organoids from Control, Control + A83-01, and Smad2/3 cKO mice (n = 6, n = 9 and n = 12, per group, respectively). RNAseq was performed in organoids from four mice per group. Histograms represent mean ± SEM analyzed by an ordinary One Way ANOVA with Tukey multiple comparison post test.