FIG. 7.

Western blot analyses of reduced E. intestinalis spore protein or infected cell lysate reacted with agarose-bound lectins and detected with either MAb 11B2 or 7G7. (A) Purified spores were reduced with 2-mercaptoethanol, run on an SDS-PAGE gel, and detected with MAbs to SWP1 (11B2) and SWP2 (7G7). (B) Cultured infected cell lysate was reacted with the agarose-bound lectin ConA or WGA with or without the inhibiting sugar (methyl-α-mannopyranoside for ConA; chitin hydrolysate for WGA).