FIG. 9.

Western blot analysis of reduced E. intestinalis spore proteins detected with sera from in vivo-infected IFN-γR⁻ mice and wild-type control mice. (A) Sera from six infected IFN-γR⁻ mice were collected on days 15, 29, 45, and 60 postinfection, pooled at each time point, and reacted with reduced E. intestinalis spore proteins in duplicate. (B) Sera from six infected wild-type control mice were collected on days 15, 29, 45, and 60 postinfection, pooled at each time point, and reacted with reduced E. intestinalis spore proteins in duplicate. The arrowheads at ≈50 kDa indicate the expected size of SWP1, and the arrowheads at ≈150 kDa indicate the expected size of SWP2. SWP1 is the bottom band of the triple banding pattern seen at about 52 kDa. Controls included sera from uninfected mice and the anti-SWP1 MAb 11B2.