Fig. 4.

Influence of MAPT haplotype and disease status on soluble and insoluble α-synuclein protein levels. Bar graphs showing the effect of MAPT haplotypes (H1/H1, H2/H2) and diseases status (PD, control) on protein levels of soluble (A) and insoluble (B) α-synuclein (α-Syn) in human brain samples of the cortex of fusiform gyrus and the cortex of cerebellum. For analysis of the MAPT haplotype effects, PD cases and controls were combined, and vice versa for analysis of the disease status. Data are protein quantity (mean ± SEM) relative to GAPDH and normalized to an inter-run calibration consisting of pooled protein samples. Two-way ANOVA (haplotype × disease status), followed by Tukey’s post-hoc test: *P < 0.05; *d, significant effect of disease. C, D Representative Western blot for soluble (C) and insoluble (D) α-Syn with GAPDH (C) and Total Protein Stain (D) as reference. Each group is represented with 1 donor in each blot. The total independent sample size is: H2/H2 Control n = 5, H2/H2 PD n = 5, H1/H1 Control n = 7, H1/H1 PD n = 9). Full length Western blots are shown in Additional file 2: Figures S3 and S4