Table 3.

Effects of MAPT haplotype and disease status on expression of candidate mRNAs in human samples of fusiform gyrus and cerebellum

mRNA	Brain region	Two-way ANOVA	Main effect of haplotype	Main effect of disease	Haplotype × disease	Direction of significant effect
		P value	P value	P value	P value
Total MAPT	ctx-fg	0.182	0.047	0.741	0.404	H1/H1 > H2/H2
	ctx-cbl	0.365	0.147	0.698	0.725	–
0N MAPT	ctx-fg	0.367	0.546	0.331	0.123	–
	ctx-cbl	0.614	0.228	0.672	0.710	–
1N MAPT	ctx-fg	0.162	0.229	0.538	0.062	–
	ctx-cbl	0.534	0.151	0.988	0.910	–
2N MAPT	ctx-fg	0.540	0.781	0.651	0.671	–
	ctx-cbl	0.155	0.703	0.196	0.130	–
3R MAPT	ctx-fg	0.290	0.752	0.541	0.300	–
	ctx-cbl	0.614	0.938	0.244	0.743	–
4R MAPT	ctx-fg	0.338	0.896	0.809	0.369	–
	ctx-cbl	0.652	0.796	0.149	0.316	–
MAPT-AS1	ctx-fg	0.617	0.822	0.201	0.600	–
	ctx-cbl	0.007	0.011	0.272	0.647	H2/H2 > H1/H1
STH	ctx-fg	0.594	0.728	0.813	0.568	–
	ctx-cbl	0.458	0.170	0.646	0.432	–
NSF	ctx-fg	0.745	0.285	0.615	0.953	–
	ctx-cbl	0.822	0.442	0.618	0.915	–
PLEKHM1	ctx-fg	0.487	0.544	0.162	0.535	–
	ctx-cbl	0.497	0.467	0.519	0.101	–
SNCA	ctx-fg	0.640	0.777	0.214	0.661	–
	ctx-cbl	0.730	0.533	0.679	0.438	–
SNCB	ctx-fg	0.325	0.242	0.159	0.874	–
	ctx-cbl	0.388	0.488	0.915	0.143	–
SNCG	ctx-fg	0.839	0.768	0.407	0.753	–
	ctx-cbl	0.916	0.556	0.923	0.626	–

Bold values indicate a significant result with P < 0.05

Main effect of haplotype was analyzed by comparison of H1/H1 (Controls + PD) versus H2/H2 (Controls + PD). Main effect of disease was analyzed by comparison of PD (H1/H1 + H2/H2) versus Controls (H1/H1 + H2/H2). The interaction haplotype × disease examined the relationship between the independent variables (haplotype + disease) on the dependent variable of mRNA expression. P values were calculated using two-way ANOVA followed by Tukey’s multiple comparisons test

ctx-fg, cortex of fusiform gyrus; ctx-cbl, cerebellum