Table 5.

Effects of MAPT haplotype and disease status on levels of tau isoforms and α-syn in the insoluble protein fraction in human samples of fusiform gyrus and cerebellum

Protein fraction	Brain region	Two-way ANOVA	Main effect of haplotype	Main effect of disease	Haplotype × disease	Direction of significant effect
		P value	P value	P value	P value
α-syn	ctx-fg	0.134	0.781	0.026	0.995	PD > Control
	ctx-cbl	0.953	0.628	0.767	0.875	–
Total tau	ctx-fg	0.150	0.243	0.099	0.587	–
	ctx-cbl	0.576	0.580	0.590	0.229	–
0N3R tau	ctx-fg	0.108	0.252	0.036	0.881	PD > Control
	ctx-cbl	0.226	0.301	0.080	0.669	–
0N4R tau	ctx-fg	0.215	0.453	0.098	0.554	–
	ctx-cbl	0.371	0.380	0.145	0.469	–
1N3R tau	ctx-fg	0.362	0.491	0.130	0.889	–
	ctx-cbl	0.385	0.226	0.298	0.375	–
1N4R tau	ctx-fg	0.031	0.062	0.043	0.289	PD > Control
	ctx-fg	0.616	0.779	0.199	0.643	–
2N3R tau	ctx-cbl	0.596	0.790	0.303	0.535	–
	ctx-fg	0.966	0.626	0.939	0.992	–
2N4R tau	ctx-cbl	0.564	0.395	0.605	0.287	–
	ctx-fg	0.299	0.079	0.803	0.651	–

Bold values indicate a significant result with P < 0.05

Main effect of haplotype was analyzed by comparison of H1/H1 (Controls + PD) versus H2/H2 (Controls + PD). Main effect of disease was analyzed by comparison of PD (H1/H1 + H2/H2) versus Controls (H1/H1 + H2/H2). The interaction haplotype × disease examined the relationship between the independent variables (haplotype + disease) on the dependent variable of protein levels. P values were calculated using two-way ANOVA followed by Tukey’s multiple comparisons test

ctx-fg, cortex of fusiform gyrus; ctx-cbl, cerebellum.