Figure 1.

Generation and identification of TNFR2-knockout MC38 and CT26 colon cancer cells using the CRISPR/Cas9 system. (A) Flow cytometry analysis of TNFR2 expression on the TNFR2 knockout (TNFR2^-/-) MC38 and CT26 cell lines vs. corresponding control W.T. cell lines. The solid line represents anti-TNFR2 antibody staining; the gray-shaded histogram represents the isotype control. (B) Real-time PCR analysis of TNFR2 derived from control and TNFR2^-/- cell lines. The data shown are representatives of at least three separate experiments with similar results.