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. 2023 Jan 22;19(4):1024–1035. doi: 10.7150/ijbs.72606

Figure 2.

Figure 2

TNFR2 deficiency impairs in vitro growth of MC38 and CT26 colon cancer cells. MC38/WT and MC38/TNFR2-/- cells were counted and planted in 96-well plates with five replicates at 2,000 cells in 100 µL medium per well. CT26/WT and CT26/TNFR2-/- cells were counted and planted in 96-well plates with five replicates at 1,500 cells in 100 µL medium per well. The proliferation of cells was determined by MTT assay at the time indicated. CT26/WT, CT26/TNFR2-/-, MC38/WT, and MC38/TNFR2-/- cells were counted and planted in 6-well plates at a density of 200 cells in 2 ml medium per well. The medium was refreshed every 3 days. This assay was terminated when two clones almost grew together, and the number of clone formations in the culture was counted. Growth curves (A-B), image (C and E), number of clone formation (D and F) of MC38/WT, MC38/TNFR2-/-, CT26/WT, and CT26/TNFR2-/- cells. Data (means ± SEM, N = 3) shown are representatives of three separate experiments with similar results. By comparison with indicated groups, *p < 0.05, **P < 0.01, ***P < 0.001.