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. 2023 Feb 13;19(4):1163–1177. doi: 10.7150/ijbs.79915

Figure 5.

Figure 5

Effect of LCN2 deletion on proinflammatory cytokines in LPS-treated RAW264.7 cells. (A) Western blot analysis of cellular lysate LCN2, 24p3R, IL-6, TNF-α, iNOS, and media LCN2 in LPS-treated RAW264.7 cells. (B) Schematic drawing of LPS-treated RAW264.7 cells medium (LTM) treatment method in LTM-treated RAW264.7 cells. (C) Western blot analysis of cellular lysate LCN2, 24p3R, IL-6, TNF-α, iNOS, and media LCN2 in LTM-treated RAW264.7 cells. (D) Western blot analysis of cellular lysate LCN2, 24p3R, IL-6, TNF-α, iNOS, and media LCN2 in recombinant LCN2 (rLCN2)-treated RAW264.7 cells. (E-F) Western blot analysis (E) and quantification (F) of cellular lysate LCN2, 24p3R, IL-6, TNF-α, iNOS, HO-1, and media LCN2 in siLCN2+LPS-treated RAW264.7 cells from three independent experiment. β-actin served as a loading control. Differences between four groups were evaluated using two-way ANOVA followed by Tukey's multiple comparisons test. *P < 0.05 versus CTL. †P < 0.05 versus LPS. All data are presented as mean ± SEM.