Figure 4.

Lactate is incorporated in cellular metabolism and displaces glucose. (A) Experimental design. Mouse CD8+ T cells were activated in the presence of either 11.1 mM [U-13C6]glucose + 40 mM NaCl, or 11.1 mM [U-13C6]glucose +40 mM sodium lactate, or 11.1 mM glucose and 40 mM [U-13C6]sodium lactate. Incorporation of labelled carbons in cellular metabolites was assessed by GC-MS at 24, 48, and 72 hours after activation. (B–D) Heatmaps showing glucose- (B, C) or lactate-derived (D) carbons incorporation into cellular metabolites at 24, 48, and 72 hours after activation, expressed as Z-scores. N = 3 individual mouse donors, each one represented by a column at each time-point.