Figure 1.

Α schematic presentation of the ancestral lignin peroxidase sequence with all amino acid residues important for the oxidization of substrates for heme Fe³⁺ binding and to the Η₂O₂ across channel, as well as for Ca²⁺ binding and the formation of disulfide bonds (upper line). The six conserved regions are designated as colored closed boxes and arrows indicate their nucleotide sequences and relative positions οn the ancestral sequence. The amino acid sequences of the conserved regions are shown at the right of each colored box and the conserved domains used for the construction of primers are boxed with blue lines.