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. 2023 Feb 27;14:1095457. doi: 10.3389/fimmu.2023.1095457

Figure 6.

Figure 6

Circ_0075723 in macrophages regulate endothelial permeability through the inhibition expression of IL-1β and IL-18 by circ_0075723/miR-155-5p/SHIP1 axis. (A) Western blot analysis of VE-cadherin in HLMVEC cultured with the supernatant for 24 h. The supernatant was collected from aforementioned-LPS-primed THP-1 stimulated with nigericin bearing altered expression of circ_0075723. Data are presented as means ± SD; significant difference was identified with Student t-tests. *p < 0.05 vs. Vector or shRNA NC; ##p < 0.01 vs. LPS/nigericin + Vector; &p < 0.05 vs. LPS/nigericin + shRNA NC. (B) Western blot analysis of VE-cadherin in HLMVEC cultured with the supernatant for 24 h. The supernatant was collected from aforementioned-LPS-primed THP-1 stimulated with nigericin bearing altered expression of circ_007572 and miR-155-5p. Data are presented as means ± SD; significant difference was identified with Student t-tests. *p < 0.05 vs. vector + mimic NC; #p < 0.05 vs. LPS/nigericin + vector + mimic NC; ##p < 0.01 vs. LPS/nigericin + vector + mimic NC; &p < 0.05 vs. LPS/nigericin + mimic NC + circ_0075723-OE. (C) Western blot analysis of VE-cadherin in HLMVEC cultured with the supernatant for 24 h. The supernatant was collected from aforementioned-LPS-primed THP-1 stimulated with nigericin bearing altered expression of miR-155-5 and SHIP1. Data are presented as means ± SD; significant difference was identified with Student t-tests. *p < 0.05 vs. vector + mimic NC; #p < 0.05 vs. LPS/nigericin + vector + mimic NC; &p < 0.05 vs. LPS/nigericin +mimic NC + SHIP1-OE.