Fig. 1.

Immunoblot analysis of α-syn in DLB brains. The brain homogenate from four DLB cases and one non-demented control case was subjected to immunoblot using the anti-alpha-synuclein antibody MJFR1, which recognizes residues 118–123 of α-syn. In each sample, 20 μg of total protein was loaded per lane. For quantitative estimation, recombinant α-syn protein at three different concentrations (25 ng, 50 ng, or 100 ng per lane) was included in the analysis. Molecular mass markers in kilodaltons were shown on the left.