Fig. 2.

RT-QuIC analysis of α-syn seeding activity in untreated or autoclaved DLB brain homogenates. DLB brain homogenates were prepared either from BA9 tissue (A, B, C, D) or BA39 tissue (E, F, G, H) of DLB1 patient. Frontal cortex homogenates from a non-demented patient (Con1) were used as a control. Brain homogenates remained untreated (A, E) or autoclaved for 20 min either at 121 °C (B, F) or 132 °C (C, G). Brain homogenates (2 μL) at indicated dilutions were used to seed α-syn RT-QuIC reactions in quadruplicate. Average ThT fluorescence values from quadruplicate wells were plotted against time (The negative replicates were taken for the average). (D, H) Protein aggregation rates (PAR) were obtained by taking the inverse of lag phase time (1/T). For reactions in which average ThT signals did not exceed the threshold values during the assay, lag phases were assigned as 50 h. Data shown represent the average values ± SD of three independent experiments.