Table 1.
Inactivation effects of autoclave treatment against α-syn or prion seeding activity.
| Disease (protein) | Case ID in this study | EBTB’s reference ID | Sex | Age at death (in years) | Brain region | α-syn concentration (μg/ml) | log10 SD50/mg brain tissue (Mean ± SD) 1) |
log10 SD50 decrease following steam sterilization (Mean ± SD) | |
|---|---|---|---|---|---|---|---|---|---|
| Untreated | 121 °C | ||||||||
| DLB (α-syn) |
DLB1 | SD26/13 | M | 65 | BA39 2,3) | 1.1 | 9.70 ± 0.35 | 7.28 ± 0.12 | 2.42 ± 0.31 |
| BA9 4) | 1.1 | 8.78 ± 0.24 | 7.12 ± 0.42 | 1.67 ± 0.42 | |||||
| DLB2 | SD19/14 | M | 60 | BA9 | 1.6 | 8.82 ± 0.13 | 7.57 ± 0.38 | 1.25 ± 0.25 | |
| DLB3 | SD50/12 | M | 61 | BA9 | 2.1 | 8.95 ± 0.35 | 7.70 ± 0.20 | 1.25 ± 0.54 | |
| DLB4 | SD24/16 | F | 86 | BA9 | 0.9 | 8.07 ± 0.13 | 7.20 ± 0.00 | 0.88 ± 0.13 | |
| CWD (prion) | C22-1 | – | M | 3 | whole Brain | – | 6.82 ± 0.13 | 5.45 ± 0.25 | 1.38 ± 0.18 |
| C22-2 | – | F | 5 | whole Brain | – | 6.32 ± 0.13 | 5.07 ± 0.38 | 1.25 ± 0.35 | |
| C22-3 | – | M | 5 | whole Brain | – | 6.57 ± 0.13 | 5.20 ± 0.25 | 1.38 ± 0.18 | |
1) Data shown represent the average values ± SD of two to three independent experiments.
2) In one of three experiments where a true end-point was not reached, we assumed that the next 10-fold dilution would have given 0 positive reactions in order to calculate the SD50 values.
3) BA39 = Brodmann area 39.
4) BA9 = Brodmann area 9.