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. Author manuscript; available in PMC: 2023 Mar 13.
Published in final edited form as: Mol Psychiatry. 2022 Feb 18;27(4):2068–2079. doi: 10.1038/s41380-022-01439-4

Fig.3: Expression of PGE2 receptors in the striatum and cell population-specific effects of PGE2 receptor stimulation.

Fig.3:

a-c. Single-molecule fluorescent in situ hybridization for PGE2 receptors in the DS. Sections through the DS of brains from wild-type C57/Bl6 male mice were processed for single molecule fluorescent in situ hybridization. Sections were labeled with probes for PGE2 receptor mRNAs, Ptger1 (a), Ptger2 (b), and Ptger4 (c) in red, and Drd1 (green), and Drd2 (cyan), as indicated, and counterstained with DAPI (gray scale). Ptger1 and Ptger2 are expressed in D1- and D2-SPNs, whereas Ptger4 is mostly in D1. Confocal microscope images, scale bar, 10 μm. d-f. RT-qPCR quantification of Ptger1 (d), Ptger2 (e), and Ptger4 (f) mRNA levels in ribosome-associated mRNA purified from the NAc or DS of D1- and D2-TRAP mice. Quantification by comparative ddCt method using Rpl19 as an internal control (arbitrary units, not comparable from one graph to the other). Note that because of gene overlap with Ptger1 we cannot exclude a contribution of Pkn1 transcripts. g. Examples of immunofluorescence of pSer235-236-rpS6 (blue) in DS sections of mice treated with vehicle (PBS) or misoprostol 30 min before sacrifice. Mice were transgenic for Drd1-tdTomato (red) and D2-TRAP (green) to identify D1- and D2-SPNs. Scale bar, 30 μm. h-i. Quantification of results as in g in D1 and D2-SPNs of NAc (h) and DS (i, n=12, 6 mice per group and 2 areas of interest per mouse). Statistical analysis, 2-way ANOVA (Supplementary Table 19), Holm-Sidak’s multiple comparisons tests, ** p<0.01, **** p<10−4.