Fig. 4.

Microtubule depolymerization leads to a stiffness dependent topographically guided migration. (A) Single cell trajectories on grooved hydrogels of different stiffness. N ​≥ ​3 experiments per condition and n ​≥ ​13 ​cells per condition. (B) Scheme depicting the quantification of cell trajectories. (C) Cells' net distances versus substrate stiffness. (D) Trajectory directionality as a function of substrate stiffness. (E) Cell trajectories on grooves of different stiffness for control cells, cells treated with nocodazole (N ​≥ ​3 experiments per condition and n ​≥ ​11 ​cells per condition) and cells treated with Y-27632 and nocodazole (N ​≥ ​2 experiments per condition and n ​≥ ​10 ​cells per condition). Cell outlines are color-coded by time. Scale bar, 50 ​μm. (F) Total displacement, (G) net distance and (H) trajectory directionality index of cells treated with nocodazole or Y-27632 and nocodazole. See Tables S11–S13 for the number of cells and experiments. Data points (Mean ​± ​SE) in (C), (D), and (F–H) were fitted as an eye-guide. Statistical significance was assessed by Tukey's tests. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)