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. 2023 Mar 13;14:1371. doi: 10.1038/s41467-023-36702-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Timeline for inoculation of NHP with 10⁶ ffu DENV2ic(2NHP), DENV4ic(2NHP) or DENV2/4EDIIic(4NHP) and blood collection for quantitative reverse transcript-polymerase chain reaction (qrt-pcr) and FRNT analysis. b Replication kinetics of DENV2/4EDII is temporally similar to parental DENV2 and DENV4. Average copies/ ml sera, as determined by qrt-pcr, is shown for DENV2, DENV4 and the four DENV2/4EDII inoculated NHP. Copies/ml was measured in triplicate for each NHP, then combined with other NHP within the treatment group This data graphed by individual NHP can be seen in supplementary Fig. 2A. Hashed line represent the limit of quantitation (LOQ) for each virus. Mean values + /- SD are shown. c DENV2/4EDII sera neutralized DENV2 and DENV4. Individual Vero-81 FRNT EC₅₀ values are shown for each NHP with a bar representing the median value. EC₅₀ values were calculated using 8 point curves performed in duplicate.