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. Author manuscript; available in PMC: 2023 Mar 13.
Published in final edited form as: Nat Neurosci. 2022 Aug 1;25(8):1020–1033. doi: 10.1038/s41593-022-01127-0

Extended Data Fig. 1. Liver-expressed apoE3 restores peripheral lipid profiles.

Extended Data Fig. 1.

a, EGFP, a surrogate of apoE expression, was detected in liver of iE3/Cre+ but not iE3/Cre− mice. Similar results were observed in at least three independent experiments. Scale bar, 50 μm. b-d, ApoE levels in the liver, plasma, and brain of iE3/Cre−, iE3/Cre+ (Cre−, n=10; Cre+, n=15), and apoE3-TR (n=8) mice were examined by ELISA. **, P<0.0001. e, The apoE was not detected by Western blotting in the brains of iE3/Cre− or iE3/Cre+ mice compared to apoE3-TR mice (n=4/group). **, P<0.0001. f, The RNA levels for apoE in the brains of iE3/Cre, apoE3-TR, iE4/Cre, and apoE4-TR mice (n=8/group) were examined by real-time PCR. **, P<0.0001. g, Total cholesterol and triglyceride levels in the plasma of iE3/Cre− or iE3/Cre+ mice (Cre−, n=9; Cre+, n=11) and apoE3-TR mice (n=8) were examined. Cholesterol: **, P<0.0001. Triglyceride: Cre− vs. Cre+: **, P=0.002; Cre− vs. E3: **, P=0.0002. h, Distribution of plasma lipoprotein cholesterol in iE3/Cre− or iE3/Cre+ mice (n=3/group) at 12 months of age. Pooled plasma from apoE3-TR mice (n=3/group) was included as control. Data represent mean ± s.e.m. One-way analysis of variance (ANOVA) with Tukey’s post-hoc test was used for statistical analyses. N.S., not significant.