KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-cFos | Millipore | Cat# abe457 |
| Rabbit anti-somatostatin-14 | Peninsula Laboratories | Cat# T-4103 |
| Mouse anti-parvalbumin | Millipore | Cat# mab1572 |
| Rabbit anti-vasoactive intestinal peptide | Immunostar | Cat# 20077 |
| Goat anti-rabbit Alexa fluor 647 | Jackson Immunoresearch | Cat# 111–605-045 |
| Goat anti-mouse Alexa fluor 647 | Jackson Immunoresearch | Cat# 115–605-003 |
| Bacterial and Virus strains | ||
| AAV1-EF1a-DIO-hChR2(H134R)-eYFP-WPRE | Karl Deisseroth Lab: Cre-activated AAV expression plasmids Unpublished |
Addgene Cat# 20298 |
| AAV1-CBA-FLEX-Arch-GFP | High-performance genetically targetable optical neural silencing by light-driven proton pumps. Chow BY, Han X, Dobry AS, Qian X, Chuong AS, Li M, Henninger MA, Belfort GM, Lin Y, Monahan PE, Boyden ES Nature. 2010 Jan 7. 463(7277):98–102. |
Addgene Cat# 22222 |
| AAV1-EF1a-DIO-eYFP-WPRE | Karl Deisseroth Lab: Double Floxed Inverted ORF Control Unpublished |
Addgene Cat# 27056 |
| AAV8-hSyn-mCherry | Karl Deisseroth Lab: mCherry Control Unpublished | Addgene Cat# 114472 |
| rAAVDJ/nEF-Creon/Flpon-hChR2(H134R)-eYFP | University of North Carolina Gene Therapy Center Vector Core | n/a |
| rAAVDJ/nEF-Creon/Flpon-eYFP | University of North Carolina Gene Therapy Center Vector Core | n/a |
| AAV8-ESARE-ERCreER-PEST | Boston Children’s Hospital Vector Core | n/a |
| Chemicals, peptides, and recombinant proteins | ||
| 4-hydroxytamoxifen (70% Z-isomer) | Sigma | Cat# H6278 |
| Morphine sulfate | Sigma | Cat# 1448005 |
| Tetrodotoxin citrate | Abcam | Cat# ab120055 |
| 4-Aminopyridine | Abcam | Cat# ab120122 |
| Cyanquixaline disodium salt | Abcam | Cat# ab120044 |
| Picrotoxin | Abcam | Cat# 120315 |
| Experimental models: Organisms/strains | ||
| Mouse: C57Bl/6J | Jackson Laboratory | Stock No. 000664 |
| Mouse: SST-FlpO | Jackson Laboratory | Stock No. 028579 |
| Mouse: PV-IRES-Cre | Jackson Laboratory | Stock No. 017320 |
| Mouse: Ai9 | Jackson Laboratory | Stock No. 007909 |
| Mouse Ai65F | Jackson Laboratory | Stock No. 032864 |
| Recombinant DNA | ||
| pFBAAV-ESARE-ERCreER-PEST | Gift from Dr. Haruhiko Bito; Kawashima et al. (2013). |
n/a |
| Software and algorithms | ||
| MedAssociates Video Freeze | MedAssociates | https://www.med-associates.com/product/video-fear-conditioning/ |
| Zen 2012 SP2 | Zeiss | https://www.zeiss.com/microscopy/us/products/microscope-software/zen.html |
| Clampex 10.3.1 | Molecular Devices | https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-oftware/pclamp-software-suite |
| NIH ImageJ | National Institutes of Health | https://imagej.nih.gov/ij/index.html |
| VLC media player 2.1.3 Rincewind | VideoLAN | https://www.videolan.org/ |
| MiniAnalysis | Synaptosoft | http://www.synaptosoft.com/MiniAnalysis/ |
| Fusion v5.6 SuperFlex | Omnitech Electronics | http://www.omnitech-electronics.com/product/Fusion-Software/510 |
| Photoshop 12.1 | Adobe | https://www.adobe.com/products/photoshopfamily.html |
| OriginPro | OriginLab | https://www.originlab.com/ |
| Prism 5 | GraphPad | https://www.graphpad.com/scientific-software/prism |
| BioRender | BioRender | https://biorender.com/ |