FIG. 1.
Complementation of the killing defect in hcnC mutant MP507. (A) Restriction map of the hcnABC region, showing the locations and orientations of known genes hcnA, hcnB, hcnC, and exoY and of putative genes (unlabeled arrows), including a homologue of the conserved hypothetical E. coli protein gene ycnB. The solid triangle indicates the location of the mTn5-Tc transposon insertion in the hcnC mutant MP507. (B and C) Maps of the insertion regions in recombinant plasmids carrying the hcnABC region. The results of nematode killing assays for hcnC mutant MP507 carrying these plasmids are also shown. The open triangles indicate the orientations of the Plac promoter in the pUCP18 vector. The killing percentages are averages based on three separate assays. MP507 carrying only the vector plasmid pUCP18 exhibited less than 1% killing.