TABLE 1.
Mutants defective in paralytic killing
| Strain | Insertion sitea | Geneb | Function | % Killingc |
|---|---|---|---|---|
| PAO1 | 99 (±0) | |||
| Class I (strongly avirulent strains) | ||||
| MP503 | 3,571,648 | eda | 2-Keto-3-deoxy-6-phosphogluconate aldolase; Entner-Doudoroff pathway | 4 (±2) |
| MP505 | 435,468 | proC | Δ-1-Pyrroline-5-carboxylate reductase; proline biosynthesis | 4 (±3) |
| MP506 | 435,230 | proC | Δ-1-Pyrroline-5-carboxylate reductase; proline biosynthesis | 0 (±0) |
| MP504 | 812,969 | PA0745d | Probably enoyl-coenzyme A hydratase; fatty acid degradation | 13 (±10) |
| MP507 | 2,415,450 | hcnC | Hydrogen cyanide synthase | 3 (±2) |
| MP508 | 45,846 | PA0041d | Homologue of fhaB, Bordetella filamentous hemagglutinin | 0 (±0) |
| MP501 | 4,423,808 | PA3946d | Homologue of bvgS, Bordetella two-component sensor kinase virulence gene regulator | 0 (±0) |
| MP502 | 1,015,249 | gacS | Homologue of Pseudomonas syringae two-component sensor kinase controlling disease lesion formation | 9 (±5) |
| MP511 | Unsequencede | 0 (±0) | ||
| Class II (moderately avirulent strains) | ||||
| MP554 | 3,572,897 | zwf | Glucose-6-phosphate dehydrogenase; Entner-Doudoroff pathway | 41 (±19) |
| MP555 | 6,098,814 | soxA | Sarcosine oxidase | 38 (±20) |
| MP556 | 6,100,002 | soxA | Sarcosine oxidase | 39 (±19) |
| MP557 | 1,032,886 | purM | Phosphoribosylaminoimldazole synthetase; purine biosynthesis | 92 (±3) |
| MP558 | 4,216,505 | purL | Phosphoribosylformylglycinamidine synthase; purine biosynthesis | 90 (±5) |
| MP559 | 874,405 | prpB | Carboxyphosphonoenolpyruvate phosphonomutase; fatty acid and phospholipid metabolism | 83 (±15) |
| MP560 | 873,168 | prpC | Citrate synthase 2 | 72 (±28) |
| MP561 | 1,758,910 | gpdA | Glycerol-3-phosphate dehydrogenase; specific to fatty acid and phospholipid metabolism | 57 (±23) |
| MP562 | 2,927,500 | PA2587 | Putative salicylate hydroxylase; quinolone signal synthesis | 27 (±16) |
| MP571 | 6,193,910 | znuB | Permease of ABC zinc transporter | 39 (±20) |
| MP573 | 4,290,026 | phpA | Aminopeptidase; protein modification, alginate biosynthesis | 28 (±13) |
| MP574 | 5,993,742 | algC | Lipopolysaccharide and alginate biosynthesis | 75 (±15) |
| MP572 | 5,100,129 | pilW | Type 4 pili | 56 (±10) |
| MP551 | 1,086,674 | PA1003d | Putative transcriptional regulator with LysR family signature | 31 (±22) |
| MP552 | 5,304,505 | PA4725d | Putative amino acid permease fused to putative two-component sensor histidine kinase | 42 (±15) |
| MP553 | 5,304,930 | PA4725d | Putative amino acid permease fused to putative two-component sensor histidine kinase | 76 (±16) |
a
The transposon insertion site corresponds to the chromosomal location in the PAO1 single contig sequence (www.pseudomonas.com).
b
Boldface type indicates known P. aeruginosa genes; lightface type indicates close homologues of known genes. PA numbers are designations assigned by the web site (www.pseudomonas.com).
c
Percentages of killing are averages based on at least three independent killing assays for each strain. The numbers in parentheses are standard errors of the means.
d
Gene not experimentally characterized in studies of pseudomonads.
e
Repeated attempts to sequence were unsuccessful.