TABLE 2.
Exoproduct production by P. aeruginosa mutants
| Strain | Mutant gene | % Killinga | Amt of cyanide (nmol)b | Production of:
|
||
|---|---|---|---|---|---|---|
| Pyocyaninc | Pyoverdinec | Proteased | ||||
| PAO1 | None | 100 (±1) | 300 (±56) | 1.00 (±0.047) | 1.00 (±0.079) | ++ |
| PAO-R1 | lasR | 0 (±0) | <10 (±0) | 0.011 (±0.000) | 1.54 (±0.013) | +/− |
| Class I (strongly avirulent strains) | ||||||
| MP503 | eda | 7 (±1) | <15 (±8) | 0.038 (±0.015) | 0.083 (±0.099) | + |
| MP505 | proC | 9 (±6) | 31 (±3) | ND | ND | ND |
| MP506 | proC | 0 | 44 (±34) | 0.608 (±0.057) | 0.303 (±0.006) | ++ |
| MP504 | PA0745 (fad-1) | 19 (±21) | <11 (±2) | 0.036 (±0.006) | 0.379 (±0.018) | ++ |
| MP507 | hcnC | 13 | <10 (±0) | 2.35 (±0.040) | 1.54 (±0.009) | ++ |
| MP508 | PA0041 (fhaB) | 0 | <10 (±0) | 1.71 (±0.059) | 0.134 (±0.035) | +++ |
| MP501 | PA3946 (bvgS) | 0 (±0) | <17 (±6) | 0.521 (±0.025) | 1.58 (±0.002) | ++ |
| MP502 | gacS | 11 | 21 (±12) | 0.127 (±0.002) | 0.064 (±0.070) | ++ |
| MP511 | Unknown | 0 | 36 (±37) | 0.034 (±0.006) | 0.033 (±0.026) | +/− |
| Class II (moderately avirulent strains) | ||||||
| MP554 | zwf | 74 (±25) | 200 (±86) | 0.254 (±0.032) | 0.447 (±0.169) | ++ |
| MP555 | soxA | 3 | 64 (±7) | 1.00 (±0.085) | 0.353 (±0.009) | ++ |
| MP556 | soxA | 17 | 55 (±15) | ND | ND | ND |
| MP562 | PA2587 | 0 (±0) | 36 (±31) | 0.013 (±0.006) | 1.03 (±0.283) | ++ |
| MP571 | znuB | 4 | 96 | 0.767 (±0.042) | 0.597 (±0.476) | ++ |
| MP573 | phpA | 14 | 150 | 1.11 (±0.021) | 0.879 (±0.046) | ++ |
| MP572 | pilW | 36 | 84 | ND | ND | ND |
| MP551 | PA1003 | 0 (±0) | <23 (±23) | 0.008 (±0.000) | 1.54 (±0.039) | ++ |
Worm killing was assayed in trials parallel to two of the three cyanide collection trials by using plates inoculated and incubated exactly like the plates used for cyanide collection. The numbers in parentheses are standard errors of the means determined when two assays were conducted.
The values are averages based on one to three independent assays. The numbers in parentheses are standard errors of the means determined when multiple assays were conducted.
The values are expressed in arbitrary units normalized to the amount in PAO1 and are averages based on two independent assays. The numbers in parentheses are standard errors of the means. ND, not done.
Total secreted protease production was assessed by determining the relative zones of clearing around same-size overnight bacterial patches on skim milk agar as follows: +/−, no clearing beyond edge of patch; +, 1 to 2 mm clearing; ++, 4 to 5 mm clearing; +++, >6 mm clearing; ND, not done.