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. 2023 Mar 13;23:138. doi: 10.1186/s12870-023-04139-5

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Bioinformatic analyses of EF genes in M. truncatula and expression pattern of MtEF1A1. (A) Phylogenetic tree shows the relationships between the 34 EF gene family members in M. truncatula. MtEF1A (MTR_6g021805) is marked by a red pentagram. (B) Analysis of conserved motifs in EF family proteins. The conserved motifs 1–10 were indicated by different colors. (C) Expression levels of MtEF1A1 of different tissues in wild-type M. truncatula (R108) and expression levels were normalized to leaf. Different lowercase letters indicate significant differences (p < 0.05). Differential expression of MtEF1A1 in response to 200 mM NaCl for 0, 1, 2, 8, 12, 24 h in M. truncatula R108 of leaf (D), stem (E) and root (F). Each tissues expression level in salt-treated was normalized to that of plants at point 0 h. Values are means ± SD of three biological replicates. Different lowercase letters indicate significant differences (p < 0.05)