Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Mar 13;21:54. doi: 10.1186/s12964-023-01076-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — Differential contribution of eIF-5A1 and eIF5-A2 to cell proliferation and migration. A A549 and H1395 cells were transfected with either a control siRNA (Control) or EIF5A1- and EIF5A2-specific siRNAs (siEIF5A1 and siEIF5A2) for 72 h. The mRNA expression was analyzed by RT-qPCR. Experimental means (n = 3 with experimental triplicates) were compared by two-way ANOVA analysis with Tukey's test for multiple comparison of samples (***p < 0.001, ****p < 0.0001). B eIF-5A1 and eIF-5A2 protein levels upon transfection of siRNA. eIF-5A1 and eIF-5A2 proteins levels were assayed by immunoblotting in A549 and H1395 cells processed as in (A). b-Actin or HSP90 were used as a loading control. A representative image of the experiments performed is shown (N = 3). C Cell proliferation was assayed using the MTS assay upon siEIF5A1 and siEIF5A2 transfection. Experimental means (n = 6 with experimental triplicates) were compared by two-way ANOVA with Tukey's test for multiple sample comparison (***p < 0.001, ****p < 0.0001).) D Analysis of cell migration in siRNA EIF5A1 or siRNA EIF5A2 transfected cells. Wound closure was performed 96 h after trasnfection and cell migration was monitored by phase-contrast microscopy. The means of the experiments (n = 3 with experimental triplicates) were compared by two-way ANOVA analysis with Tukey's test for multiple comparison of samples (**p < 0.01, ***p < 0.001, ****p < 0.0001) (left panel). Phase constrast representatives images. Scale bar 500 µm. E Genetic inhibition of EIF5A1 and EIF5A2 alters the organization of the F-actin citoeskeleton. Representative fluorescence microscopy images of A549 and H1395 cells transfected with siEIF5A1 and siEIF5A2 for 72 h. F-actin cytoskeleton was stained with rhodamine phalloidin (red). Dapi staining (blue) was used to visualised the nuclei. Scale bar of A549 fluorescence images 25 µm; scale bar of H1395 fluorescence images 10 µm