Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Mar 13;21:54. doi: 10.1186/s12964-023-01076-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 3 — Overexpression of eIF-5A2 induces cell proliferation and migration, and enhances translation. A Protein extracts from empty vector (H1395-EV) or Flag-eIF-5A2 (H1395-eIF-5A2) stably-transfected H1395 cells were analysed by western blot with eIF-5A2 and Flag antibodies. HSP90 was used as a loading control protein expression. A representative image of the three idependent experiments is shown. B Cell proliferation assay in H1395-EV and H1395-eIF-5A2 cells. MTS assays were performed 24 h, 48 h and 72 h after seeding the cells. Experimental means (n = 6 with experimental triplicates) were compared by two-way ANOVA with Tukey's test for multiple sample comparison (**p < 0.01). C Cell migration assay in H1395-EV and H1395-eIF-5A2 cells. Wound was performed in confluent monolayer cell culture, and wound closure was monitored by phase-contrast microscopy. The means of the experiments (n = 3 with experimental triplicates) were compared by two-way ANOVA analysis with Tukey's test for multiple comparison of samples (*p < 0.05) (right panel). Representative images of phase contrast microscopy are shown in the left panel. Scale bar 500 µm. D Polysomal profile of H1395-EV and H1395-eIF-5A2 cells. Global RNA polysome profiles generated by measurement of the OD at 254 nm in the density gradient fractionation system are shown. From left to right, each peak represents the 40S subunit, the 60S subunit, monosomes (80S), disomes, and polysomes. A representative graph of the experiments performed is shown (n = 3). E Protein extracts from the fractions obtained in D were analysed by western blot with eIF-5A2, RPL4 and rpS6 antibodies. A representative image of the experiments performed is shown (n = 2)