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. 2023 Mar 13;21:54. doi: 10.1186/s12964-023-01076-6

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — TGFB1 induces eIF-5A2 protein expression. A EIF5A1 and EIF5A2 mRNA levels in A549 and H1395 cells treated with TGFB1. Cells were transfected with siEIF5A1, siEIF5A2 or siCT for 72 h and treated or not with TGFβ1 for 48 h. The mRNA relative expression was analyzed by RT-qPCR. Experimental means (n = 3 with experimental triplicates) were compared by two-way ANOVA analysis with Tukey's test for multiple comparison of samples (*p < 0.05, **p < 0.01, ****p < 0.0001). B eIF-5A2 and eIF-5A1 protein levels of cells in A were analised by western blot. β-Actin or HSP90 were used as a loading control. A representative image of the experiments performed is shown (n = 3)