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. 2023 Mar 13;21:54. doi: 10.1186/s12964-023-01076-6

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — TGFB1 induces eIF-5A2 hypusination and eIF-5A2-dependent expression of EMT proteins. A EIF5A1 and EIF5A2 mRNA levels in H1395-EV and H1395-eIF-5A2 cells treated with TGFB1 for the indicated times. The mRNA expression was analyzed by RT-qPCR. Experimental means (n = 3 with experimental triplicates) were compared by two-way ANOVA analysis with Tukey's test for multiple comparison of samples (*p < 0.05, **p < 0.01, ***p < 0.001). B eIF-5A1 and eIF-5A2 protein levels and their hypsuinated forms of cells in A were analysed by western blot with eIF-5A2 and hypusine antibodies. HSP90 was used as a loading control. A representative image of the experiments performed is shown (n = 3). C mRNA levels of FN1, FHOD1, EZRIN and SNAI1 of cells in A. The mRNA expression levels were analyzed by RT-qPCR. Experimental means (n = 3 with experimental triplicates) were compared by two-way ANOVA analysis with Tukey's test for multiple comparison of samples (*p < 0.05, **p < 0.01). D Protein expression of Fibronectin, FHOD1, Ezrin and SNAI1 of cells in A were analysed by western blot with the indicated antibodies. A representative image of the experiments performed is shown (n = 3). E Cell migration assays in H1395-EV and H1395-eIF-5A2 cells, in the presence of TGFB1 ligand for 72 h. Wound was performed in confluent monolayer cell culture, and wound closure was monitored by phase-contrast microscopy. The means of the experiments (n = 3 with experimental triplicates) were compared by two-way ANOVA analysis with Tukey's test for multiple comparison of samples (*p < 0.05) (left panel). Representative phase-contrast microscopy images are shown in the right panel. Scale bar 500 µm